I just remembered of one episode in Wednesday, where she unleashes piranhas into the pool full of bullies, causing the leader to lose one of his testicles. It's not castration, but it's a awareness of the impotence a man gets when his manhood is affected. I don't know if this scene was already mentioned in this thread.
https://www.youtube.com/watch?v=9UtfMM7-b4Q